Pnxgate32-3ha

Author: mkai

August undefined, 2024

WebPNXGATE32-3HA : plasmid : High copy Split Ubiquitin vector for fusing proteins with the NubG peptide. The resulting fusions contain NubG at the N-terminus and a tripple HA tag … WebAug 17, 2016 · ThyAP4 cells expressing empty pNXgate32-3HA + pMetYC vectors, BjNRAMP4.1, BjHCF164 or BjNRAMP4.1 + BjHCF164 were spotted on (-M-L-W) medium …

Stock Detail

WebThis gene is a variant of pTorPE-Y-GECO1 (Plasmid #55765) Addgene NGS results found F261V in the araC translation. The depositing laboratory believes that this is not a problem for araC or plasmid function WebDec 10, 2016 · The full-length HSP21 protein fused to the NubG fragment of the pNXgate32-3HA vector was used as the prey. The full-length mature subunits of D1, D2, CP47, PsaA and PsaB fused to the LexA-VP16-Cub fragment of the pNCW vector were used as bait. is isagenix collagen worth the money

Two plastidic glycolate/glycerate translocator 1 isoforms …

Web(i) The pNXgate33-3HA is a low-copy CEN plasmid, which mediates significantly lower expression of NubG-ORF fusions when compared to pNXgate32-3HA. Therefore the use … Web1 introduction and general information 3 1.1 abbreviations 4 1.2 mbsus package contents 5 1.3 references and citation 6 1.4 the mbsus in brief 7 1.4.1 general information 7 1.4.2 … WebPNXGATE32-3HA: plasmid: High copy Split Ubiquitin vector for fusing proteins with the NubG peptide. The resulting fusions contain NubG at the N-terminus and a tripple HA tag … isis agriculture

The N-Terminal Extension Domain of the - PLOS

Pnxgate32-3ha

Detecting interactions with membrane proteins using a

WebSep 16, 2024 · The cDNA library was shuttled into pNXgate32 3HA with inserted ccdB gene. Transformation of highly competent E. coli (NovaXG Zappers, Novagen, Germany) … WebZestimate® Home Value: $831,400. 3 Northgate Dr, Ponte Vedra Beach, FL is a single family home that contains 2,037 sq ft and was built in 1983. It contains 2 bedrooms and 2 …

Did you know?

WebDescription: Control construct for split ubiquitin system in pNXgate32-3HA vector. Growth Requirement: grow in LB media at 37C for 12 to 24 hours Marker: ampicillin Background: ABRC Comment: Format Shipped: bacterial stab Base / Commercial Price: $13 / $104 WebObrdlik P, El Bakkoury M, Hamacher T, Cappellaro C, Vilarino C, Fleischer C, et al.K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions.

WebJul 24, 2024 · Catalog Number : 105076 Product Name : pNXgate32-3HA article : bacterial resistance : Ampicillin cloning : backbone pNXgate32-3HA backbone_mutation … Web实验步骤. 酵母双杂交系统正是利用了GAL4的功能特点，通过两个杂交蛋白在酵母细胞中的相互结合及对报告基因的转录激活来捕获新的蛋白质，其大致步骤为: 1、构建重组Activation Domain（AD）融合的重组质粒（pGADT7载体）以及Binding Domain（BD）融合的重组质粒 …

WebJul 24, 2024 · pNXgate32-3HA. catalog : 105076. more info or order : Addgene product webpage. citations: 1. Reference; Obrdlik P, El Bakkoury M, Hamacher T, Cappellaro C, Vilarino C, Fleischer C, et al. K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions. Webname type description ; PNXGATE32-3HA: plasmid: High copy Split Ubiquitin vector for fusing proteins with the NubG peptide. The resulting fusions contain NubG at the N-terminus and a tripple HA tag at the C-terminus.

WebUSB ports on the device can be used for a variety of purposes. The primary use for the USB ports is to install or reinstall the operating system on the device. Beyond that, there are …

WebHover: Reveals additional information for enzyme cut sites, ORFs, feature descriptions, and primer details. Select: Click one location on a nucleotide and then click on a second nucleotide location. The region between the two locations will be highlighted. The sequence clipboard box will appear with the top strand sequence of the selected region. keppel road cafesWebDownload Publication - Boyce Thompson Institute - Cornell University keppel pacific oak share priceWebpMetYCgate, pNXgate32-3HA have been described in detail (Grefen et al., 2007). The vector pMetYCgate is used to create C-terminal Cub fusion proteins and the vector pNXgate32 … is isaiah 24 apocalypticWebJul 1, 2010 · The CDS of OsPLGG1b was cloned into pNXgate32-3HA to produce Nub-OsPLGG1b prey plasmids. The bait and prey vectors were transformed into the yeast strains THY.AP4 and THY.AP5, ... keppel offshore \u0026 marine malaysiaWebControl construct for split ubiquitin system in pNXgate32-3HA vector. Growth Requirement: grow in LB media at 37C for 12 to 24 hours. Marker: ampicillin. Background: ABRC … is isaiah 30:25 about judgment or blessingWebSep 23, 2010 · For NubG fusions, pNXgate32/33-3HA or pXNgate21-3HA vectors were cleaved with EcoRI/SmaI, whereas pMetYCgate vector was cleaved with PstI/HindIII. Gel-purified PCR products and linearized vectors were co-transformed into THY.AP4 or THY.AP5 strains for creating CubPLV bait and NubG prey clones, respectively. THY.AP4 cells … keppel power promotionWebFeb 1, 2010 · The vector pMetYCgate is used to create C-terminal Cub fusion proteins and the vector pNXgate32-3HA is used to create N-terminal Nub fusion proteins. Following … keppel road eastham