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Phospho irf3 antibody

WebIntracellular Delivery of Lipopolysaccharide Induces Effective Th1-Immune Responses Independent of IL-12 Sachiko Watanabe1., Joe Inoue1,2*. 1Department of Biosciences, School of Science and Graduate School of Science, Kitasato University, Japan, 2Department of Immunology and Cell Biology, Graduate WebWestern Blot analysis of Phospho-IRF3 (Ser386) in HeLa cells using a Phospho-IRF3 (Ser386) Polyclonal antibody ( Product # PA5-121307) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer 3% BSA was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution.

Radiation Dose Escalation Is Crucial in Anti-CTLA-4 Antibody …

WebIRF3 Antibody detects endogenous levels of total IRF3 protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthtic peptide corresponding to a residues surrounding proline 307 of human IRF-3. Antibodies are purified by protein A and peptide affinity chromatography. WebFigure Legend Snippet: TAOK1 promotes TBK1-IRF3 complex formation. a HEK293T cells were transfected with plasmids encoding HA-TBK1, Flag-IRF3, and varying doses of a plasmid encoding Myc-TAOK1 (0.5, 1.0, and 1.5 μg). Cells were harvested 24 h after transfection for immunoblot analysis as indicated with the antibody to Flag tag. b … dannenberg company macon georgia https://e-healthcaresystems.com

Anti-phospho-IRF-3 (Ser396) Antibody 07-582-I - EMD Millipore

WebMouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C. ... Rat Heart tissue lysates; Lane 4: Rat Testis tissue lysates probed with Phospho-IRF3 (Ser396) Polyclonal Antibody ... WebDuring infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and of a kinase complex that includes IKKε and TBK1 (6,7). IRF-3 is … WebAnti-IRF3 antibody (ab25950) Datasheet Reviews ( 3) Q&A (1) References (33) Key features and details Rabbit polyclonal to IRF3 Suitable for: WB Reacts with: Human Isotype: IgG You may also be interested in Western … birthday gifts for ladies uk

Difficulty detecting human phospho-IRF3 on Western Blot

Category:Phospho-IRF3-S396 Rabbit pAb-Phospho-specific Antibodies

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Phospho irf3 antibody

anti-Homo sapiens (Human) Phospho-IRF3 (S386) Antibody

WebApr 23, 2024 · Cyclic GMP-AMP synthase (cGAS) plays an important role in biological responses to pathogens. The activation of the cGAS pathway in immune cells is known to induce antitumor effects, but the role of cGAS in cancer cells remains poorly understood. In silico analysis using public databases suggested that high cGAS expression in head and … WebAnti-Phospho-IRF3-Ser385 antibody (320-400) (STJ91040) Western blot analysis of lysates from HT29 cells treated with INSULIN 0.01U/ML 15', using IRF-3 (Phospho-Ser385) Antibody. The lane on the right is blocked with the phospho peptide. Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRF-3 (Phospho-Ser385) Antibody.

Phospho irf3 antibody

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WebPhospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb (PE Conjugate) recognizes endogenous levels of IRF-3 protein only when phosphorylated at Ser396. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser396 of human IRF-3 protein. WebPrimary Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859) Protein Recombinant Human IRF3 protein (ab205216) View more associated products …

WebThe wells are washed and rabbit anti-phospho-IRF3 (S386) antibody is used to detect phosphorylated IRF3. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IRF3 (S386) bound. WebJun 1, 2024 · IRF-3 Antibody (SL-12) is an IgG 1 κ mouse monoclonal IRF-3 antibody (also designated IRF3 antibody) that detects the IRF-3 protein of mouse, rat and human origin …

WebUse Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3. Anti-phospho … WebPhospho-IRF-3 (Ser386) (E7J8G) XP ® recognizes endogenous levels of IRF-3 protein only when phosphorylated at Ser386. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser386 of human IRF-3 protein. Background

WebPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation. The antibody was purified by affinity chromatography. Concentration. 0.5 mg/ml. Storage & Handling. The antibody solution should be stored undiluted between 2°C and 8°C. Application. WB - Quality tested.

http://www.gbwpt.com/382818.html danner 6 kinetic gore-tex waterproof bootWebThe phospho-IRF3 (Ser386) assay measures IRF3 when phosphorylated at Ser386. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The phospho-IRF3 (Ser386) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. birthday gifts for law school studentsWebMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser396 of human IRF-3. Validations Biological Strategies Applications Western Blotting (WB) Reactivity Homo sapiens (Human) Mus musculus (House mouse) View additional product information Experiments from the … dannen quarter top hiking bootbirthday gifts for kpop fansWebICC/IF analysis of PFA-fixed HepG2 cells (30min of 4μM Forskolin treatment) using GTX02883 IRF3 (phospho Ser396) antibody. Red : Primary antibody Green : Beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100 GTX02883 IHC-P Image IHC-P analysis of mouse heart tissue using GTX02883 IRF3 (phospho Ser396) antibody. ... birthday gifts for kids boyWebBlot results show a ~50kDa IRF3 band that is detected by total IRF3 antibody (abcam 68481). None-specific bands occur for both total IRF3 and phospho IRF3 at 35 and 50kDa. I'm not sure... birthday gifts for leo manWebThis antibody is predicted to react with Monkey. Target Information IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent … birthday gifts for lady that only works