WebMay 1, 2024 · SWI/SNF chromatin remodeling complexes regulate gene transcription, DNA replication and DNA repair by organizing the chromatin architecture via ATP hydrolysis (1). There are three defined members of this subfamily: the canonical BRG-/BRM-associated factor (BAF), polybromo-associated BAF complexes and a newly identified non-canonical … WebDec 30, 2024 · Cisplatin goes a step further than a simple alkylating agent though, because it has another reactive site and can thus crosslink (covalently bond) another nucleotide, possibly on another strand of DNA, making a strong obstruction to DNA replication. Cisplatin can also crosslink proteins to DNA.
Cisplatin 14. DNA - Chemistry LibreTexts
WebReactivation of drug-treated plasmids, observed in four cell lines, suggests that oxaliplatin adducts are repaired with similar kinetics as cisplatin adducts. Oxaliplatin, however, was more efficient than cisplatin per equal number of DNA adducts in inhibiting DNA chain elongation ( approximately 7-fold in CEM cells). WebFeb 7, 2024 · DNA lesions arising from both exogenous and endogenous sources occur frequently in DNA. During DNA replication, the presence of unrepaired DNA damage in the template can arrest replication fork progression, leading to fork collapse, double-strand break formation, and to genome instability. To facilitate completion of replication and … ts5 homes for sale
7.5: DNA Lesions - Biology LibreTexts
WebCisplatin is a chemotherapy medication used to treat a number of cancers. These include testicular cancer, ovarian cancer, cervical cancer, breast cancer, bladder cancer, head and neck cancer,... WebSep 9, 2014 · This therefore demonstrates that they do not absolutely hinder DNA replication. They may however affect replicative enzyme performance and accuracy. Although PRR takes place primarily during cell replication, cisplatin resistant cell lines show an activity during non-replication, therefore indicating that it may be involved in cisplatin … WebHere, we have applied Damage-seq to construct a human genome DNA damage map for cisplatin-induced damage. In Damage-seq (Fig. 1A and SI Appendix, Fig. S1), cells were treated with cisplatin and the genomic DNA was isolated and fragmented by sonication. Then the fragment ends were repaired, followed by ligation of the first adapter. phillip tom memorial pages